David Ehrhardt

Department of Plant Biology
Carnegie Institution for Science
260 Panama Street
Stanford, CA 94305
Phone: (650) 325-1521 x261
Fax: (650) 325-6857 
Email: ehrhardt stanford.edu

 


Plant Cell Development
We investigate plant cell development and morphogenesis, utilizing live cell imaging techniques and moelcular genetics  to visualize and investigate the organization and dynamic behaviors of molecules and organelles. Problems of special interest include how cells generate asymmetries and specific shapes. A current focus is how the cortical microtubule cytoskeleton acquires organization and how this organization in turn functions to organize the plasma membrane and cell wall to guide patterns of cell growth and division.

Ehrhardt lab website  

 
Selected Publications:
 
Cartwright H, Ehrhardt DW. Effective elimination of plastid and cell wall autofluorescence in plant tissue by fluorescence emission time-gating. In prep.
 
Grossman G, Lindeboom J, Frommer W, Ehrhardt DW. The cortical microtubule array acts as an anisotropic diffusion barrier in the plasma membrane of Arabidopsis. In prep.
 
Gutierrez R, Kirik V, Ehrhardt DW. Dynamic visualization of SEC1 reveals linear microdomains for secretion defied by cortical microtubules and cellulose biosynthesis. In prep.
 
Lindeboom J, Nakamura M, Hibbel A, Sedbrook J, Walia A. Kettelar T, Kirik V, Mulder B and Ehrhardt DW. A crucial role for microtubule rescue by CLASP in reorientation of Arabidopsis cortical arrays. In prep.
 
Lindeboom J, Nakamura M, Hibbel A, Hashimoto T, Mulder B and Ehrhardt DW. SPR2 is a minus end tracking protein essential for cortical array reorientation in Arabidopsis. In prep.
 
Provart NJ, Alonso J, Assmann SM, Bergmann D, Brady SM, Brkljacic J, Browse J, Chapple C, Colot V, Cutler S, Dangl J, Ehrhardt D, Friesner JD, Frommer WB, Grotewold E, Meyerowitz E, Nemhauser J, Nordborg M, Pikaard C, Shanklin J, Somerville C, Stitt M, Torii KU, Waese J, Wagner D, McCourt P. (2017) 50 years of Arabidopsis research: highlights and future directions. New Phytol. 2016 Feb;209(3):921-44. doi: 10.1111/nph.13687.
 
Watanabe Y, Meents MJ, McDonnell LM, Barkwill. Sampathkumar, SA, Cartwright HN, Demura T, Ehrhardt DW, Samuels L, Mansfield SD. (2015) Visualization of Cellulose Synthases in Arabidopsis Secondary Cell Walls. Science. In press.
 
Worden N, Wilkop TE, Esteve VE, Jeannotte R, Lathe R, Vernhettes S, Weimer B, Hicks G, Alonso J, Labavitch J, Persson S, Ehrhardt D, Drakakaki G. (2015) CESA trafficking inhibitor inhibits cellulose deposition and interferes with the trafficking of cellululose synthase complexes and their associated proteins KORRIGAN and POM2/CSI1. Plant Physiol. 2015 Feb;167(2):381-93. doi: 10.1104/pp.114.249003. Epub 2014 Dec 22.
 
Walia A, Nakamura M, Kirik V, Hashimoto T, Ehrhardt DW. (2014) GCP-WD mediates γ-TuRC recruitment and the geometry of microtubule-dependent microtubule nucleation in Arabidopsis. Curr Biol. 2014 Nov 3;24(21):2548-55. doi: 10.1016/j.cub.2014.09.013. Epub 2014 Oct 16.
 
Galva C, Kirik V, Lindeboom JL, Kaloriti D, Rancour DM, Hussey PJ, Bednarek SY, Ehrhardt DW, and Sedbrook JC. (2014) The Microtubule Plus-End Tracking Proteins SPR1 and EB1b Interact to Maintain Polar Cell Elongation and Directional Organ Growth in Arabidopsis. Plant Cell Nov;26(11):4409-25. doi: 10.1105/tpc.114.131482. Epub 2014 Nov 18.
 
Denninger P, Bleckmann A, Lausser A, Vogler F, Ott T, Ehrhardt DW, Frommer WB, Sprunck S, Dresselhaus T, Grossmann G. (2014) Male-female communication triggers calcium signatures during fertilization in Arabidopsis. Nat Commun. 2014 Aug 22;5:4645. doi:10.1038/ncomms5645.
 
Lipka E, Gadeyne A, Stöckle D, Zimmermann S, De Jaeger G, Ehrhardt DW, Kirik V, Van Damme D, Müller S. (2014) The phragmoplast-orienting kinesin-12 class proteins translate the positional information of the pre-prophase band to establish the cortical division zone in Arabidopsis thaliana. Plant Cell. 2014 Jun 27;26(6):2617-2632.
 
Khanna R, Li J, Tseng T-S, Schroeder JI, Ehrhardt DW, Briggs WB. (2014) COP1 Jointly Modulates Cytoskeletal Processes and Electrophysiological Responses Required For Stomatal Closure. Molecular Plant. 7(9):1441-54. doi: 10.1093/mp/ssu065. Epub 2014 May 23.
 
Ehrhardt DW, Bezanilla M. (2013) Patterning the cell: membrane-cytoskeletal crosstalk. Curr Opin Plant Biol. doi: 10.1016/j.pbi.2013.10.014. Epub 2013 Nov 19.
 
Lindeboom J, Nakamura M, Hibbel A, Shundyak K, Gutierrez R, Ketelaar T, Emons A-M, Mulder B, Kirik V, Ehrhardt DW. A mechanism for reorientation of cortical microtubule arrays driven by microtubule severing. Science. doi: 10.1126/science.1245533. Epub 2013 Nov 7.
 
Riely, BK, Larrainzar E, Haney CH, Mun J-H, Gil-Quintana E, González EM, Yu H- J, Tricoli D, Ehrhardt DW, Long SR, and Cook DR (2013) Development of Tools for the Biochemical Characterization of the Symbiotic Receptor-Like Kinase DMI2. Molecular Plant-Microbe Interactions. 26:2, 216-226
 
Sampathkumar A, Gutierrez R, McFarlane H, Bringmann M, Lindeboom, J Emons A- M, Samuels L, Ketelaar T, Ehrhardt DW, Persson. (2013) Patterning and life-time of plasma membrane localized cellulose synthase is dependent on actin organization in Arabidopsis interphase cells. Plant Physiology. Plant Physiol. doi: 10.1104/pp.113.215277. Epub 2013 Apr 19.
 
Lin D, Cao L, Liu G, Zhou Z, Zhu L, Ehrhardt DW, Yang Z, Fu Y. 2013) Rho GTPase coordinates severing with nucleation of cortical microtubules in the promotion of their alignment and order in Arabidopsis. Current Biology. Curr Biol. 2013 Feb 18;23(4):290-7. doi: 10.1016/j.cub.2013.01.022. Epub 2013 Feb 7.
 
Lindeboom JL, Lioutas A, Deinum E, Tindemans S, Ehrhardt DW , Emons A-M, Vos JW, Mulder BL. (2013) Cortical microtubule arrays are initiated from a non-random pre- pattern driven by atypical microtubule initiation. Plant Physiol. 2013 Mar;161(3):1189- 201. doi: 10.1104/pp.112.204057. Epub 2013 Jan 8.
 
Shaw SL, Ehrhardt DW. Smaller, faster, brighter: Advances in optical imaging of living plant cells. (2013) Annu Rev Plant Biol. 2013;64:351-75. doi: 10.1146/annurev- arplant-042110- 103843. Epub 2013 Mar 13. Review.
 
Huang KC, Ehrhardt DW, Shaevitz J (2013) The molecular origins of chiral growth in walled cells. Trends in Cell Biology. Curr Opin Microbiol. 2012 Dec;15(6):707-14.
doi: 10.1016/j.mib.2012.11.002. Epub 2012 Nov 26. Review.
 
Eisinger W, Ehrhardt DW, Briggs WR. (2012) Quantitative changes in microtubule distribution correlate with guard cell function in Arabidopsis. Molecular Plant. 5:716-25.
 
Eisinger W, Ehrhardt DW, Briggs WR. (2012) Microtubules are Essential for Guard Cell Function in Vicia and Arabidopsis. Molecular Plant. 5:601-10.
 
Nakamura M, Yagi M, Kato T, Fujita S, Kawashima N, Ehrhardt DW and Hashimoto T. (2012) Arabidopsis MOZART1 is an integral component of the γ-tubulin-containing microtubule nucleating complex. The Plant Journal. 71:216-25.
 
Kirik A, Ehrhardt DW, and Kirik V. (2012) TON2/FASS regulates the geometry of microtubule nucleation and cortical array organization in interphase plant cells. Plant Cell. 2012 Mar 6. [Epub ahead of print]
 
Harris, DM, Corbina K, Wang T, Gutierrez R, Bertolod AL, Pettia C, Smilgiese D-M, Estevez JM, Bonetta D, Urbanowicz BR, Ehrhardt DW, Somerville CR, Rose JKC, Hong M, and DeBolt S (2012) Cellulose microfibril crystallinity is reduced by mutating C- terminal transmembrane region residues CESA1A903V and CESA3T941I of cellulose synthase. Proc Natl Acad Sci. Mar 13;109(11):4098-103. Epub 2012 Feb 28.
 
Ehrhardt DW and Frommer WB. (2012) New technologies for 21st century plant science. PlantCell. Feb;24(2):374-94. Epub 2012 Feb 24.
 
Nakamura M, Ehrhardt DW1, Hashimoto T1. (2010) Microtubule and katanin dependent dynamics of microtubule nucleation complexes in the acentrosomal Arabidopsis cortical array. Nature Cell Biol. Published ahead of print October 11, 2010. doi 038/ncb2110.
 
Chen S, Ehrhardt DW, Somerville CR  (2010) Mutations of cellulose synthase (CESA1) phosphorylation sites modulate anisotropic cell expansion and bidirectional mobility of cellulose synthase. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17188-93. Epub 2010 Sep 20.
 
Opportunities to explore plant membrane organization with super-resolution microscopy. (2010) Gutierrez R, Grossmann G, Frommer WB, Ehrhardt DW. Plant Physiol. 2010 Oct;154(2):463-6.
 
Gutierrez R, Lindeboom J, Paredez AR, Emons AM and Ehrhardt DW. (2009) The microtubule cortical array organizes cellulose synthase trafficking. Nat Cell Biol. 2009 Jul;11(7):797-806. Epub 2009 Jun 14.
 
Paredez AR, Persson S, Ehrhardt DW, Somerville CR. (2008) Genetic evidence that cellulose synthase activity influences microtubule cortical array organization.  Plant Physiol. 2008 Aug;147(4):1723-34. Epub 2008 Jun 26.
 
Ying-Lang Wan, William Eisinger, David Ehrhardt, Ulrich Kubitscheck, Frantisek Baluska, and Winslow Briggs (2008) The Subcellular Localization and Blue-Light-Induced Movement of Phototropin 1-GFP in Etiolated Seedlings of Arabidopsis thaliana. Mol Plant 1: 103-117.
 
Lalonde S, Ehrhardt DW, Loqué D, Chen J, Rhee SY, Frommer WB (2008) Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations. Plant J. 53(4):610-35.
 
Ehrhardt DW (2008) Straighten up and fly right: microtubule dynamics and organization of non-centrosomal arrays in higher plants. Curr Opin Cell Biol. 20(1):107-16.
 
Kirik V, Herrmann U, Parupalli C, Sedbrook JC, Ehrhardt* DW, Hülskamp* M (2007) CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis. J Cell Sci. 120(Pt 24):4416-25. Epub 2007 Nov 27. *co-PI’s.
 
Silady RA, Ehrhardt DW, Jackson K, Faulkner C, Oparka K, Somerville CR. (2007) The GRV2/RME-8 protein of Arabidopsis functions in the late endocytic pathway and is required for vacuolar membrane flow. Plant J. 53(1):29-41. Epub 2007 Oct 27.
 
Walker KL, Müller S, Moss D, Ehrhardt DW, Smith LG (2007) Arabidopsis TANGLED identifies the division plane throughout mitosis and cytokinesis. Curr Biol. 17(21):1827-36. Epub 2007 Oct 25.
 
DeBolt S, Gutierrez R, Ehrhardt DW, Somerville C. (2007) Nonmotile cellulose synthase subunits repeatedly accumulate within localized regions at the plasma membrane in Arabidopsis hypocotyl cells following 2,6-dichlorobenzonitrile treatment. Plant Physiol. 145(2):334-8.
 
DeBolt S., Gutierrez R., Ehrhardt D.W., Melo C.V., Ross L., Cutler S.R., Somerville C.R., and Bonetta, D. (2007) Morlin, an inhibitor of cortical microtubule dynamics and cellulose synthase movement. PNAS. published March 27, 2007, 10.1073/pnas.0700789104
 
Paredez, A, Wright A.,  Ehrhardt D.W. (2006) Microtubule cortical array organization and plant cell morphogenesis. Curr Opin Plant Biol. 2006 Dec;9(6):571-8. Epub 2006 Sep 28.
 
Paredez, A., Somerville CR, Ehrhardt DW. (2006) Dynamic visualization of cellulose synthase demonstrates functional association with cortical microtubules. Science. Published online April 20 2006; 10.1126/science.1126551.
 
Shaw SR, Ehrhardt DW. (2006) Microtubule dynamics and organization in the plant cortical array. Annu Rev Plant Biol. 2006;57:859-75.
 
Koh S, Andre A, Edwards H, Ehrhardt D, Somerville S. (2005) Arabidopsis thaliana subcellular responses to compatible Erysiphe cichoracearum infections. Plant J. 44(3):516-29.
 
Lalonde S, Ehrhardt DW, Frommer WB. (2005) Shining light on signaling and metabolic networks by genetically encoded biosensors. Curr Opin Plant Biol. 8(6):574-81. Epub 2005 Sep 26.
 
Assaad FF, Qiu JL, Youngs H, Ehrhardt D, Zimmerli L, Kalde M, Wanner G, Peck SC, Edwards H, Ramonell K, Somerville CR, Thordal-Christensen H. (2004) The PEN1 syntaxin defines a novel cellular compartment upon fungal attack and is required for the timely assembly of papillae. Mol Biol Cell. 15(11):5118-29.
 
Sedbrook JC, Ehrhardt DW, Fisher SE, Scheible WR, Somerville CR. (2004) The Arabidopsis sku6/spiral1 gene encodes a plus end-localized microtubule-interacting protein involved in directional cell expansion. Plant Cell. 16:1506-20. Epub 2004 May 21.
 
Tian GW, Mohanty A, Chary SN, Li S, Paap B, Drakakaki G, Kopec CD, Li J, Ehrhardt D, Jackson D, Rhee SY, Raikhel NV, Citovsky V. (2004) High-throughput fluorescent tagging of full-length Arabidopsis gene products in planta. Plant Physiol. 2004 135:25-38.
 
Shaw S.L., Kamyar R. and Ehrhardt D.W. (2003) Sustained Microtubule Treadmilling in Arabidopsis Cortical Arrays.  Science. Published online 24 April 2003; 10.1126/science.1083529.
 
Ehrhardt D. (2003) GFP technology for live cell imaging. Curr Opin Plant Biol. 2003 6:622-8
 
Cutler S.R. and Ehrhardt D.W. (2002) Polarized cytokinesis in vacuolate cells of Arabidopsis.. Proc. Natl. Acad. Sci. 99: 2812-2817.
 
Cutler S.R., Ehrhardt D.W. (2000) Dead cells don't dance: insights from live-cell imaging in plants. Curr Opin Plant Biol. 2000 Dec;3(6):532-537.
 
Cutler S.R., Ehrhardt D.W., Somerville C.R. (2000) Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. Proc. Natl. Acad. Sci. 97:3718-3723.