Phosphate Sensor Data
Variant
Recognition element
FRET Pair
Apo ratio*
Delta ratio
Delta ratio (%)
Kd (µM)
E. coli
Addgene
FLIPPi-260n
E. coli
ECFP-Venus
1.76
-0.13
-7
AddGene pRSET-B link
FLIPPi-770n
E. coli
ECFP-Venus
2.62
-1.07
-41
pRSET-B
FLIPPi-4u
E. coli
ECFP-Venus
3.05
-1.34
-44
Addgene pRSET-B link
FLIPPi-5u
E. coli
ECFP-Venus
3.1
-1.33
-43
Addgene pRSET-B link
FLIPPi-200u
E. coli
ECFP-Venus
3.22
-1.13
-35
Addgene pRSET-B link
FLIPPi-30m
E. coli
ECFP-Venus
3.01
-1.03
-34
Addgene pRSET-B link