We are generating a genome-wide collection of mutants with known mutation sites in Chlamydomonas.
This is the first such collection in any single-celled photosynthetic eukaryote.
The goal of this NSF-funded project is to isolate two mutants for 80% of all genes. We are leveraging novel high-throughput insertion site mapping and robotic mutant propagation strategies developed in our lab. The mutants will be distributed to the community through the Chlamydomonas Resource Center.
The PI on the project is Martin Jonikas. Other PI's involved in the project are Arthur Grossman at Carnegie (Co-PI on the grant- advising on all aspects of the project), Pete Lefebvre at the University of Minnesota (who will oversee distribution of the resource to the community), and Eva Huala at Carnegie (developing the mutant distribution website).
The daily operations of the project are led by Xiaobo Li. Also working on the project are: Weronika Patena, Ru Zhang, Spencer Gang, Sean Blum, Nina Ivanova, Rebecca Yue.