Biological systems function through interactions of proteins with other macromolecules (proteins, DNA, RNA, complex carbohydrates and lipids), and with small molecules, including metabolites and secondary compounds. Membranes provide a surface for perceiving and transducing signals from adjacent cells and external conditions such as availability of nutrients, or toxins and adverse conditions for growth and development. In addition, membranes control cellular and subcellular entry and exit of molecules. To understand the regulation of cell-environment, cell-cell, and intracellular compartment to cytosol interactions better, we plan to determine the interactions of 8,400 proteins from the reference plant Arabidopsis. This set comprises essentially integral membrane proteins (~4,300) and a large number (>1,000) of proteins predicted to be involved in signaling or protein modification. 

Read more...