Title: Finding a kinase for phosphorylation of Phosphoenolpyruvate Carboxykinase altering enzyme activity for gluconeogenic reactions
Abstract: Phosphoenolpyruvate carboxykinase 1 (PCK1) is the key gene of gluconeogenesis which catalyzes the step from oxaloacetic acid to phosphoenolpyruvate. PCK1 is common role in animal and plant. Phosphorylated PCK1 is inactive in various plant species but it’s still unclear what the phosphorylation of PCK1 alters its activity in Arabidopsis or not.
The summer intern student would be involved in the following aspects: 1) Examine PCK1 activity in Arabidopsis after light treatment. 2) Carry out in vitro kinase assay to determine whether the candidate kinase catalyze the phosphorylation on PCK1. 3) Assess PCK1 degradation under the phosphorylated or dephosphorylated state. I am preparing plant materials and constructs to perform the above-mentioned experiments.