Ribose Sensor Data
Variant
Recognition element
FRET Pair
Apo ratio*
Delta ratio
Delta ratio (%)
Kd (µM)
E. coli
Mammalian cells
order from Addgene
FLIPrib-250n
E. coli
ECFP-EYFP
0.19
0.25±0.3
pRSET-B
pcDNA3.1
FLIPrib-400n
E. coli
ECFP-EYFP
0.3
pRSET-B
pcDNA3.1
FLIPrib-4µ
E. coli
ECFP-EYFP
0.36
4.29±0.26
pRSET-B
pcDNA3.1
FLIPrib-120µ
E. coli
ECFP-EYFP
0.29
pRSET-B
pcDNA3.1
FLIPrib-3m
E. coli
ECFP-EYFP
0.37
2900±310
pRSET-B
pcDNA3.1
FLIPrib-12m
E. coli
ECFP-EYFP
0.38
11700±480
pRSET-B
pcDNA3.1