Tryptophan Sensor Data
Variant
Recognition element
FRET Pair
Apo ratio
Delta ratio
Delta ratio (%)
Kd (µM)
E. coli
Mammalian cells
FLIPW-CTY
E. coli TrpR
ECFP-Venus
4.1
-0.41
-10
FLIPW-TCTY
E. coli TrpR
ECFP-Venus
1.57
-0.03
-2
FLIPW-CTYT
E. coli TrpR
ECFP-Venus
2.08
0.35
17
FLIPW-CTTY
E. coli TrpR
ECFP-Venus
2.85
n.d*
n.d*
*not determinable