Glutamate Sensor Data
Variant
Recognition element
FRET Pair
Apo ratio*
Delta ratio
Delta ratio (%)
Kd (µM)
E. coli
Yeast
Mammalian cells
Plants
Addgene pRSET-B
Addgene pcDNA
Addgene pDisplay
cytosol
plasma membrane
Cytosol
FLIPE-600n
E.coli
ECFP-Venus
2.0
1.7
-14
pDRf1GW
CF203
Addgene pRSET-B link AddGene pcDNA link AddGene pDisplay link
FLIPE-10µ-1
E.coli
ECFP-Venus
1.6
0.7
-56
pDRf1GW
CF203
Addgene pRSET-B link AddGene pcDNA link AddGene pDisplay link
FLIPE-10µ-2
E.coli
ECFP-Venus
5.8
1.7
-71
pRSETB
CF203
FLIPE-100µ
E.coli
ECFP-Venus
1.4
1.2
-13
pDRf1GW
Addgene pRSET-B link AddGene pcDNA link AddGene pDisplay link
FLIPE-1m
E.coli
ECFP-Venus
1.3
1.2
-10
pDRf1GW
CF203
Addgene pRSET-B link AddGene pcDNA link AddGene pDisplay link
FLIPE-1m-2
E.coli
ECFP-Venus
2.0
1.5
-25
pRSETB
FLII81E-1µ
E.coli
ECFP-Venus
2.0
1.0
-50
1
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
FLII81E-10µ
E.coli
ECFP-Venus
1.6
0.8
-52
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203
FLII81E-1m
E.coli
ECFP-Venus
2.0
1.0
-50
pRSETB
pDRf1GW
pcDNA3.1
pDisplay
CF203