Sucrose Sensor Data
Variant
Recognition element
FRET Pair
Apo ratio*
Delta ratio
Kd (µM)
E. coli
FLIPsuc-4µ
A. thumefaciens ThuE
ECFP-EYFP
0.18
pRSET-B
FLIPsuc-10µ
A. thumefaciens ThuE
ECFP-EYFP
0.12
pRSET-B
FLIPsuc-30µ
A. thumefaciens ThuE
ECFP-EYFP
0.5
30
pRSET-B
FLIPsuc-90µ
A. thumefaciens ThuE
ECFP-EYFP
0.28
FLIPsuc-160µ
A. thumefaciens ThuE
ECFP-EYFP
0.5
160
pRSET-B
FLIPsuc-1m
A. thumefaciens ThuE
ECFP-EYFP
0.68
pRSET-B
FLIPsuc-3m
A. thumefaciens ThuE
ECFP-EYFP
0.36
pRSET-B
FLIPsuc-15m
A. thumefaciens ThuE
ECFP-EYFP
0.14
pRSET-B
FLIPsuc-46m
A. thumefaciens ThuE
ECFP-EYFP
0.40
pRSET-B